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专利名称：NUCLEIC ACID ADAPTORS AND USES
THEREOF
发明人：Bin LI,Zhoutao CHEN,Tanya BIORAC,Melvin
WEI
申请号：US15372938申请日：20161208
公开号：US201700883A1公开日：20170330
摘要：Provided herein are compositions, systems, methods, and kits for joiningtogether the ends of one or more polynucleotides using at least one pair of blockingoligonucleotide adaptors. Blocking oligonucleotide adaptors can be used to reduce theformation of adaptor dimers or trimers (or higher-order concatemers) which can improvethe yield of desirable polynucleotide-adaptor products in any recombinant nucleic acidworkflow. Blocking oligonucleotide adaptors can comprise a double-stranded
oligonucleotide adaptor (duplex) having an overhang cohesive portion that anneals with ablocking oligonucleotide which can be a separate single-stranded oligonucleotide. Ablocking oligonucleotide, when annealed to an overhang portion, can preventundesirable hybridization of the overhang portion to another nucleic acid, such as theoverhang portion from another blocking oligonucleotide adaptor or a polynucleotide ofinterest.
申请人：LIFE TECHNOLOGIES CORPORATION
地址：Carlsbad CA US
国籍：US
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